A strain of the genera Pseudomonas genera was found to possess hemolytic, fibrinolytic and thrombolytic activities. The fibrinolytic activity of the lyophilized unpurified preparation was 900 conventional units/mg. After incubation in the blood plasma, the activity completely remained. The preparation (1 microgram/ml, 750 micrograms of protein) obtained by precipitation with ammonium sulfate (80% saturation) completely lysed in vitro human blood thrombi for 50 min. The strain studied can find practical applications in medical industry.