A highly fluorescent nucleoside analog based on thieno[3,4-d]pyrimidine senses mismatched pairing

Org Biomol Chem. 2008 Apr 21;6(8):1334-8. doi: 10.1039/b801054d. Epub 2008 Mar 10.

Abstract

A highly emissive nucleobase analog, based on a thieno[3,4-d]pyrimidine core, is enzymatically incorporated into RNA oilgonucleotides that function as base discriminating fluorescent probes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Pair Mismatch*
  • DNA / chemistry
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Nucleosides / chemical synthesis
  • Nucleosides / chemistry*
  • Oligonucleotides / chemistry*
  • RNA / chemistry*
  • Sensitivity and Specificity
  • Time Factors

Substances

  • 7-ribofuranosylthieno(3,4-d)pyrimidine-3,6-dione
  • Fluorescent Dyes
  • Nucleosides
  • Oligonucleotides
  • RNA
  • DNA