Two new bis-coumarin glycosides from Daphne giraldii NITSCHE

Chem Pharm Bull (Tokyo). 2008 Apr;56(4):589-91. doi: 10.1248/cpb.56.589.

Abstract

Two new bis-coumarin glycosides were isolated from the stem barks of Daphne giraldii NITSCHE. Their structures were elucidated as 6-O-alpha-L-rhamnnopyranosyl daphnogirin (1), and 6-O-beta-D-apiofuranosyl daphnogirin (2), on the basis of detailed spectroscopic analysis. Compounds 1 and 2 were tested inhibitory effects against production of nitric oxide (NO) in RAW264.7 cells, and cytotoxicity of human tumor cell lines A549, LOVO, QGY-7703, 6T-CEM. The results showed that compounds 1 and 2 neither reduced production of NO, nor inhibited human tumor cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry*
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Carbohydrate Conformation
  • Cell Line, Tumor
  • Coumarins / chemistry*
  • Coumarins / pharmacology*
  • Daphne / chemistry*
  • Drug Screening Assays, Antitumor
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / pharmacology*
  • Glycosides / chemistry*
  • Glycosides / pharmacology*
  • Humans
  • Hydrolysis
  • Macrophages / drug effects
  • Macrophages / enzymology
  • Magnetic Resonance Spectroscopy
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Plant Bark / chemistry
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet
  • Tetrazolium Salts
  • Thiazoles

Substances

  • 6-O-alpha-L-rhamnnopyranosyl daphnogirin
  • 6-O-beta-D-apiofuranosyl daphnogirin
  • Antineoplastic Agents, Phytogenic
  • Coumarins
  • Enzyme Inhibitors
  • Glycosides
  • Tetrazolium Salts
  • Thiazoles
  • Nitric Oxide Synthase
  • thiazolyl blue