Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 1;867(1):8-14. doi: 10.1016/j.jchromb.2008.02.019. Epub 2008 Feb 29.

Abstract

Rapid and precise method for the determination of 8-iso-prostaglandin F(2alpha), an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope dilution, immunoseparation combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was determined as <9.6% (0-250pg of 8-iso-prostaglandin F(2alpha)/ml EBC). The limit of detection (LOD) was 1 pg/ml EBC and limit of quantification (LOQ) 5 pg/ml EBC. A significant difference in 8-iso-prostaglandin F(2alpha) content between the group of asbestosis patients and healthy volunteers was found.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Breath Tests*
  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Dinoprost / analogs & derivatives*
  • Dinoprost / analysis
  • Humans
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • 8-epi-prostaglandin F2alpha
  • Dinoprost