Polyploidy and haploid are widely employed in the studies of genetics and evolution, and great progress has been made in these fields, inspiring the enthusiasm of scientists to explore the ploidy effects in gene expression. In this paper, we review the gene expression and its regulation in polyploids, especially in autopolyploids. We summarize some limitations in previous reports on polyploidy gene expression and its regulation, especially the limitations in the research materials. We propose an idea to create homologous ploidy series with twin-seedlings and to employ high-throughput techniques to investigate the polyploidy transcriptome and its regulation.