PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever

J Clin Microbiol. 2008 May;46(5):1861-6. doi: 10.1128/JCM.00109-08. Epub 2008 Mar 26.

Abstract

PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Antigens, Bacterial / genetics
  • Bacteremia / microbiology
  • Blood / microbiology*
  • Child
  • Child, Preschool
  • Genes, Bacterial
  • Humans
  • Paratyphoid Fever / diagnosis*
  • Paratyphoid Fever / microbiology
  • Polymerase Chain Reaction / methods*
  • Salmonella paratyphi A / genetics
  • Salmonella paratyphi A / isolation & purification*
  • Salmonella paratyphi B / genetics
  • Salmonella paratyphi B / isolation & purification*
  • Salmonella typhi / genetics
  • Salmonella typhi / isolation & purification*
  • Sensitivity and Specificity
  • Typhoid Fever / diagnosis*
  • Typhoid Fever / microbiology

Substances

  • Antigens, Bacterial