This study evaluates the outcomes of a theory-based, couple-focused HIV prevention program for Latino adolescent mothers and their male partners. The sample includes 49 couples (98 individuals) who receive either the intervention or only an HIV information session (comparison). The six-session, culturally appropriate intervention was developed through a community-academic partnership. Findings at the 6-month evaluation show that the probability of unprotected sex is significantly reduced and intentions to use condoms increase over time for participants in the experimental group, compared with the comparison group (p < .001), although AIDS knowledge improves for participants in both groups. Females in both groups have higher intentions of using condoms (p < .01) and lower probability of unsafe sex (p < .05) at baseline and over time, compared with their male partners. The intervention is well accepted by the inner-city adolescents and is realistic for implementation in a community setting.