Rhodamine-based fluorogenic probe for imaging biological thiol

Bioorg Med Chem Lett. 2008 Apr 1;18(7):2246-9. doi: 10.1016/j.bmcl.2008.03.014. Epub 2008 Mar 10.

Abstract

We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in biological conditions. Moreover, the probe was successfully applied to the imaging of thiol species in living human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzenesulfonates / chemistry
  • Cell Line
  • Cysteine / chemistry
  • Diagnostic Imaging / methods*
  • Fluorescein / chemical synthesis*
  • Fluorescent Dyes / chemical synthesis*
  • Glutathione / chemistry
  • Humans
  • Models, Chemical
  • Rhodamines / chemistry*
  • Spectrometry, Fluorescence
  • Sulfhydryl Compounds / chemistry*

Substances

  • Benzenesulfonates
  • Fluorescent Dyes
  • Rhodamines
  • Sulfhydryl Compounds
  • rhodamine 110
  • Glutathione
  • Cysteine
  • Fluorescein