Optimum diagnostic assay and clinical specimen for routine rotavirus surveillance

J Clin Microbiol. 2008 May;46(5):1842-3. doi: 10.1128/JCM.02329-07. Epub 2008 Mar 19.

Abstract

Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child, Preschool
  • Feces / virology
  • Humans
  • Immunoenzyme Techniques / methods
  • Infant
  • Infant, Newborn
  • Mass Screening / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / diagnosis*
  • Sensitivity and Specificity
  • Virology / methods*