CD3-induced T-cell proliferation and interleukin-2 secretion is modulated by the CD45 antigen

Scand J Immunol. 1991 Nov;34(5):531-7. doi: 10.1111/j.1365-3083.1991.tb01576.x.

Abstract

In the present study we have investigated the effect of CD45, CD45RA and CD45RO monoclonal antibodies (MoAbs) on the CD3 receptor-mediated proliferation of human T lymphocytes. It is shown that CD3-induced proliferation of purified resting T cells and quiescent T lymphoblasts (QTL) is promoted via all of the investigated CD45-associated epitopes. It is also shown that the CD45 molecules are required to be cross-linked for costimulation. The MoAbs enhance the interleukin-2 (IL-2) production of CD3-stimulated QTL. The elevation of the IL-2 production correlates with the increase in CD3-induced cell proliferation suggesting that the CD45-driven regulation of T lymphocyte activation is linked to the IL-2 pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD / physiology*
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • CD3 Complex
  • Histocompatibility Antigens / physiology*
  • Humans
  • Interleukin-2 / biosynthesis*
  • Leukocyte Common Antigens
  • Lymphocyte Activation*
  • Mice
  • Phosphorylation
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / immunology*
  • Tyrosine / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • Histocompatibility Antigens
  • Interleukin-2
  • Receptors, Antigen, T-Cell
  • Tyrosine
  • Leukocyte Common Antigens