A plate holder for non-destructive testing of mesophase crystallization assays

J Agirre; A Mechaly; A Cabo-Bilbao; D M A Guérin

doi:10.1007/s00249-008-0296-x

A plate holder for non-destructive testing of mesophase crystallization assays

Eur Biophys J. 2008 Jul;37(6):871-7. doi: 10.1007/s00249-008-0296-x. Epub 2008 Mar 11.

Authors

J Agirre¹, A Mechaly, A Cabo-Bilbao, D M A Guérin

Affiliation

¹ Unidad de Biofísica (CSIC-UPV/EHU), Bilbao, Spain.

PMID: 18330553
DOI: 10.1007/s00249-008-0296-x

Abstract

Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization / methods*
Crystallography / methods*
Materials Testing / methods*
Phase Transition
Proteins / chemistry*
Proteins / ultrastructure*
Specimen Handling / methods*
X-Ray Diffraction / methods*

Substances

Proteins