Abstract
Surface display is a powerful technique that uses natural microbial functional components to express proteins or peptides on the cell exterior. Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. Cells with surface display are used as biocatalysts, biosorbents and biostimulants. Microbial cell-surface display has proven to be extremely important for numerous applications, ranging from combinatorial library screening and protein engineering to bioremediation and biofuels production.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Review
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biotechnology / methods*
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Butanols / metabolism
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Combinatorial Chemistry Techniques / methods
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Environmental Restoration and Remediation / methods*
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Ethanol / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gram-Negative Bacteria / genetics
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Gram-Negative Bacteria / metabolism
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Gram-Positive Bacteria / genetics
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Gram-Positive Bacteria / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Protein Engineering / methods
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Yeasts / genetics
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Yeasts / metabolism
Substances
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Bacterial Proteins
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Butanols
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Fungal Proteins
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Membrane Proteins
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Recombinant Proteins
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Ethanol