[Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua]

Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(21):2227-31.
[Article in Chinese]

Abstract

Objective: Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.

Method: A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.

Result: The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.

Conclusion: The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Artemisia annua / enzymology
  • Artemisia annua / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / classification
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phylogeny
  • Plant Proteins / genetics*
  • Plants, Medicinal / enzymology
  • Plants, Medicinal / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Plant Proteins
  • Cytochrome P-450 Enzyme System

Associated data

  • GENBANK/DQ667171