Increasing voltage gradient electrophoresis of DNA

Daniele Oliva; Sabina Di Maio; Giorgia Sisino; Daniele Bellavia; Rainer Barbieri

doi:10.1016/j.chroma.2008.02.033

Increasing voltage gradient electrophoresis of DNA

J Chromatogr A. 2008 Apr 11;1187(1-2):205-8. doi: 10.1016/j.chroma.2008.02.033. Epub 2008 Feb 14.

Authors

Daniele Oliva¹, Sabina Di Maio, Giorgia Sisino, Daniele Bellavia, Rainer Barbieri

Affiliation

¹ Istituto di Biomedicina e Immunologia Molecolare del CNR, Palermo, Italy.

PMID: 18304558
DOI: 10.1016/j.chroma.2008.02.033

Abstract

We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / isolation & purification*
DNA, Mitochondrial / isolation & purification
Electrophoresis, Agar Gel / instrumentation
Electrophoresis, Agar Gel / methods*

Substances

DNA, Mitochondrial
DNA