Abstract
Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amphotericin B / pharmacology
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Antifungal Agents / pharmacology*
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Blotting, Northern
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Cell Wall / chemistry
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Cell Wall / drug effects
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Drug Resistance, Fungal*
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Electron Transport / drug effects*
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Electron Transport / genetics
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Flow Cytometry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Glucan Endo-1,3-beta-D-Glucosidase / metabolism
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Kluyveromyces / cytology
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Kluyveromyces / drug effects*
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Kluyveromyces / genetics
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Kluyveromyces / physiology*
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Microbial Sensitivity Tests
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Mitochondria / drug effects
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Mitochondria / genetics
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Mitochondria / physiology
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Multienzyme Complexes / metabolism
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Mutation*
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Peptide Hydrolases / metabolism
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Sterols / metabolism
Substances
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Antifungal Agents
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Fungal Proteins
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Multienzyme Complexes
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Sterols
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lyticase
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Amphotericin B
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Glucan Endo-1,3-beta-D-Glucosidase
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Peptide Hydrolases