Comparison of the positive and negative ion electrospray ionization and matrix-assisted laser desorption ionization-time-of-flight mass spectra of the reaction products of phosphatidylethanolamines and hypochlorous acid

Grit Richter; Celestina Schober; Rosmarie Süss; Beate Fuchs; Claudia Birkemeyer; Jürgen Schiller

doi:10.1016/j.ab.2008.01.029

Comparison of the positive and negative ion electrospray ionization and matrix-assisted laser desorption ionization-time-of-flight mass spectra of the reaction products of phosphatidylethanolamines and hypochlorous acid

Anal Biochem. 2008 May 1;376(1):157-9. doi: 10.1016/j.ab.2008.01.029. Epub 2008 Jan 31.

Authors

Grit Richter¹, Celestina Schober, Rosmarie Süss, Beate Fuchs, Claudia Birkemeyer, Jürgen Schiller

Affiliation

¹ University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany.

PMID: 18295587
DOI: 10.1016/j.ab.2008.01.029

Abstract

Phosphatidylethanolamines (PEs) react with HOCl under formation of the mono- and dichloramines which are easily converted into secondary products (nitriles and imines). PEs with unsaturated acyl residues also give chlorhydrines. The aim of this study was to investigate whether all products may be detected by electrospray ionization (ESI) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Results indicated that chloramines and imines are nearly exclusively detectable by ESI-MS, whereas all other products are detectable by both MALDI and ESI-MS. Therefore, ESI-MS is superior for the detection of chlorinated products of PEs.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Hypochlorous Acid / chemistry*
Phosphatidylethanolamines / chemistry*
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Phosphatidylethanolamines
phosphatidylethanolamine
Hypochlorous Acid