Upregulation of annexin A2 in H(2)O(2)-induced premature senescence as evidenced by 2D-DIGE proteome analysis

Exp Gerontol. 2008 Apr;43(4):353-9. doi: 10.1016/j.exger.2008.01.003. Epub 2008 Jan 18.

Abstract

Human diploid fibroblasts undergo premature senescence after treatment with sublethal concentration of H(2)O(2). We report the first proteomic study of microsomal proteins in the context of H(2)O(2)-induced premature senescence by using 2D-DIGE approach. Twelve different proteins with altered abundance at day 3 after treatment with H(2)O(2) were identified. Among them, we demonstrated a re-localization of annexin A2 in plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / drug effects
  • Aging / metabolism*
  • Aging, Premature / chemically induced
  • Aging, Premature / metabolism
  • Annexin A2 / drug effects
  • Annexin A2 / metabolism*
  • Diploidy
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Oxidants / pharmacology
  • Proteins / drug effects
  • Proteins / metabolism*
  • RNA, Messenger / metabolism
  • Up-Regulation

Substances

  • ANXA2 protein, human
  • Annexin A2
  • Oxidants
  • Proteins
  • RNA, Messenger
  • Hydrogen Peroxide