Abstract
Human diploid fibroblasts undergo premature senescence after treatment with sublethal concentration of H(2)O(2). We report the first proteomic study of microsomal proteins in the context of H(2)O(2)-induced premature senescence by using 2D-DIGE approach. Twelve different proteins with altered abundance at day 3 after treatment with H(2)O(2) were identified. Among them, we demonstrated a re-localization of annexin A2 in plasma membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging / drug effects
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Aging / metabolism*
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Aging, Premature / chemically induced
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Aging, Premature / metabolism
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Annexin A2 / drug effects
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Annexin A2 / metabolism*
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Diploidy
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Electrophoresis, Gel, Two-Dimensional / methods
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Humans
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Hydrogen Peroxide / pharmacology
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Oxidants / pharmacology
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Proteins / drug effects
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Proteins / metabolism*
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RNA, Messenger / metabolism
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Up-Regulation
Substances
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ANXA2 protein, human
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Annexin A2
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Oxidants
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Proteins
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RNA, Messenger
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Hydrogen Peroxide