Abstract
Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Arabidopsis / genetics
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Arabidopsis Proteins / genetics
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Dimerization
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Light*
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Phytochrome B / genetics
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Protein Processing, Post-Translational
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Protein Splicing / radiation effects*
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Recombinant Fusion Proteins / genetics
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae* / genetics
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Saccharomyces cerevisiae* / radiation effects
Substances
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Arabidopsis Proteins
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Basic Helix-Loop-Helix Transcription Factors
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PIF3 protein, Arabidopsis
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Phytochrome B