Objective: To study the extract process of Cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba.
Methods: CAMP was extracted with water, separated and purificated by ion exchange and silica gel G column chromatography.
Results: Thin layer chromatography (TLC) of the extracts and control cAMP showed the same Rf 0.75; The UV-absorption of the extracts and control cAMP had maximal absorb peak at 260 nm and the least absorb value at 230 nm; Infra-red spectrum of the extracts was indistinguishable from that of control cAMP; Retention time of the extracts by high-performance liquid chromatography (HPLC) determinate was 5.237 minute,unsettled impurity peak,content was 97%. Control cAMP was 5.350 minute,their retention time were of little difference.
Conclusion: Chemistry structure of this extract with that of control cAMP is the same.