Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17

Yuliya P Fedonenko; Olga N Konnova; Evelina L Zdorovenko; Svetlana A Konnova; George V Zatonsky; Alexander S Shashkov; Vladimir V Ignatov; Yuriy A Knirel

doi:10.1016/j.carres.2007.12.013

Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17

Carbohydr Res. 2008 Mar 17;343(4):810-6. doi: 10.1016/j.carres.2007.12.013. Epub 2007 Dec 25.

Authors

Yuliya P Fedonenko¹, Olga N Konnova, Evelina L Zdorovenko, Svetlana A Konnova, George V Zatonsky, Alexander S Shashkov, Vladimir V Ignatov, Yuriy A Knirel

Affiliation

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.

PMID: 18226805
DOI: 10.1016/j.carres.2007.12.013

Abstract

A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17. The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy, including a 2D NOESY experiment: [Formula: see text] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum brasilense / chemistry*
Carbohydrate Sequence
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data

Substances

Lipopolysaccharides