Parasitological examination of stool samples of 9867 individuals, submitted from both inpatient and outpatient departments to the Microbiology Department of an 800-bed Training Hospital between 1.1.2003 and 31.12.2006, revealed that 582 individuals (5.9%) were infected with intestinal parasites. Different diagnostic methods were employed in two different periods, and Blastocystis hominis, Giardia intestinalis and Entamoeba histolytica/dispar were found to be the most common parasites, respectively. Patients were predominantly male (67% (n=336) male vs. 33% (n=165) female) and aged between 15-25 years (36%). In addition, 14% (81/582) patients had multiple parasitoses. Despite being labor-intensive, parasitological examination of stool samples with necessary staining methods by experienced staff will surely help determine both the diagnosis and exact prevalence of intestinal parasitic infections in Turkey.