Abstract
The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Cell Line, Tumor
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Cell Nucleus / metabolism
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Drug Resistance, Multiple / drug effects
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Drug Resistance, Multiple / genetics*
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Drug Resistance, Neoplasm / genetics*
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Gene Expression Regulation, Neoplastic / drug effects
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Microscopy, Fluorescence
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Protein Transport / drug effects
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Vinblastine / pharmacology
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Y-Box-Binding Protein 1 / genetics
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Y-Box-Binding Protein 1 / metabolism
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Y-Box-Binding Protein 1 / physiology*
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Recombinant Fusion Proteins
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Y-Box-Binding Protein 1
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Green Fluorescent Proteins
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Vinblastine