Development of drug resistance in the population of colon cancer cells under the effect of multifunctional protein YB-1

Bull Exp Biol Med. 2007 Apr;143(4):463-6. doi: 10.1007/s10517-007-0157-0.

Abstract

The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
  • Cell Line, Tumor
  • Cell Nucleus / metabolism
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology
  • Drug Resistance, Multiple / drug effects
  • Drug Resistance, Multiple / genetics*
  • Drug Resistance, Neoplasm / genetics*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Microscopy, Fluorescence
  • Protein Transport / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Vinblastine / pharmacology
  • Y-Box-Binding Protein 1 / genetics
  • Y-Box-Binding Protein 1 / metabolism
  • Y-Box-Binding Protein 1 / physiology*

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Recombinant Fusion Proteins
  • Y-Box-Binding Protein 1
  • Green Fluorescent Proteins
  • Vinblastine