Near-infrared fluorescent labeled peptosome for application to cancer imaging

Bioconjug Chem. 2008 Jan;19(1):109-17. doi: 10.1021/bc7001665. Epub 2007 Dec 29.

Abstract

Nonionic amphiphilic copolypeptides, which were composed of hydrophilic poly(sarcosine) and hydrophobic poly(gamma-methyl L-glutamate) blocks, were synthesized with varying chain lengths of the blocks. The polypeptides having a suitable hydrophilic and hydrophobic balance were found to form vesicular assemblies of 100 nm size in buffer, which was evidenced by the TEM observation, the DLS analysis, and the encapsulation experiment. The genuine peptide vesicles, peptosomes, were labeled with a near-infrared fluorescence (NIRF) probe. In vivo retention in blood experiment showed long circulation of the peptosome in rat blood as stable as the PEGylated liposome. NIRF imaging of a small cancer on mouse by using the peptosome as a nanocarrier was successful due to the EPR effect of the peptosome. Peptosome is shown here as a novel excellent nanocarrier for molecular imaging.

MeSH terms

  • Animals
  • Circular Dichroism
  • Drug Carriers / metabolism
  • Fluorescein-5-isothiocyanate / chemistry
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry
  • Infrared Rays*
  • Male
  • Mice
  • Micelles
  • Nanostructures / chemistry
  • Neoplasms / metabolism*
  • Peptides / analysis*
  • Peptides / chemistry
  • Permeability
  • Polyglutamic Acid / analogs & derivatives
  • Polyglutamic Acid / chemistry
  • Rats
  • Sarcosine / analogs & derivatives
  • Sarcosine / chemistry

Substances

  • Drug Carriers
  • Fluorescent Dyes
  • Micelles
  • Peptides
  • poly-gamma-methylglutamate
  • Polyglutamic Acid
  • polysarcosine
  • Fluorescein-5-isothiocyanate
  • Sarcosine