Optimization of cross-linking parameters during production of transglutaminase-hardened spherical multinuclear microcapsules by complex coacervation

Colloids Surf B Biointerfaces. 2008 May 1;63(1):41-7. doi: 10.1016/j.colsurfb.2007.11.007. Epub 2007 Nov 21.

Abstract

Gelatin-gum arabic spherical multinuclear microcapsules (SMMs) encapsulating peppermint oil were prepared by complex coacervation. Transglutaminase (TG) was used to harden the SMMs by complex coacervation instead of traditional reagents such as formaldehyde or glutaraldehyde. The effect of various cross-linking parameters on the hardening effectiveness of SMMs containing peppermint oil was investigated. The optimum parameters were as follows: hardening for 6h at 15 degrees C and pH 6.0 with a TG concentration of 15 U/g gelatin. Compared with formaldehyde, TG exhibits similar microcapsule hardening effectiveness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsules*
  • Cross-Linking Reagents
  • Excipients
  • Formaldehyde / chemistry
  • Gelatin
  • Gum Arabic
  • Hydrogen-Ion Concentration
  • Mentha piperita
  • Particle Size
  • Plant Oils / administration & dosage
  • Plant Oils / chemistry
  • Temperature
  • Transglutaminases / chemistry*
  • Triglycerides / chemistry
  • Water

Substances

  • Capsules
  • Cross-Linking Reagents
  • Excipients
  • Plant Oils
  • Triglycerides
  • Water
  • Formaldehyde
  • Gum Arabic
  • Gelatin
  • peppermint oil
  • Transglutaminases