Paramagnetic relaxation-based 19f MRI probe to detect protease activity

J Am Chem Soc. 2008 Jan 23;130(3):794-5. doi: 10.1021/ja077058z. Epub 2007 Dec 23.

Abstract

A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caspase 3 / analysis
  • Fluorine / chemistry*
  • Humans
  • Isotopes / chemistry
  • Molecular Probes / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry*
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Peptide Hydrolases / analysis*

Substances

  • Isotopes
  • Molecular Probes
  • Oligopeptides
  • Organometallic Compounds
  • gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid 4-trifluoromethoxybenzylamide
  • Fluorine
  • Peptide Hydrolases
  • Caspase 3