Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification

Arch Virol. 2008;153(2):337-42. doi: 10.1007/s00705-007-1092-0. Epub 2007 Dec 17.

Abstract

Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-gamma) or endosomal- lysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 +/- 39% increase), IFN-gamma (446 +/- 8%), ammonium chloride + IFN-gamma (1721 +/- 283%), chloroquine diphosphate (1037 +/- 121%), chloroquine diphosphate + IFN-gamma (2199 +/- 255%), monensin (950 +/- 178%) and monensin + IFN-gamma (1948 +/- 60%). Combined IFN-gamma and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonium Chloride / pharmacology
  • Animals
  • Cell Line / drug effects
  • Chloroquine / analogs & derivatives
  • Chloroquine / pharmacology
  • Circovirus / growth & development*
  • Endosomes / drug effects*
  • Enzyme Inhibitors / pharmacology*
  • Immunologic Factors / pharmacology*
  • Interferon-gamma / pharmacology*
  • Monensin / pharmacology
  • Swine

Substances

  • Enzyme Inhibitors
  • Immunologic Factors
  • Ammonium Chloride
  • chloroquine diphosphate
  • Interferon-gamma
  • Chloroquine
  • Monensin