A faster and more accurate assay for intracellular replication of Legionella pneumophila in amoebae hosts

David A Coil; Jozef Anné; Elke Lammertyn

doi:10.1016/j.mimet.2007.11.009

A faster and more accurate assay for intracellular replication of Legionella pneumophila in amoebae hosts

J Microbiol Methods. 2008 Feb;72(2):214-6. doi: 10.1016/j.mimet.2007.11.009. Epub 2007 Nov 21.

Authors

David A Coil¹, Jozef Anné, Elke Lammertyn

Affiliation

¹ Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit, Leuven, Minderbroedersstraat 10, Leuven, Belgium.

PMID: 18077026
DOI: 10.1016/j.mimet.2007.11.009

Abstract

A faster, more sensitive, and more accurate method to study intracellular replication of Legionella pneumophila in amoeboid hosts is described. This assay relies on an automated plate-reader to examine the intracellular growth and release of bacteria in real-time. Our experiments using this method have already revealed new insights into the kinetics of intracellular replication of L. pneumophila in Acanthamoeba castellanii.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Amoebida / microbiology*
Animals
Bacteriological Techniques / methods*
Legionella pneumophila / genetics
Legionella pneumophila / physiology*
Reproducibility of Results
Spectrophotometry / methods