NS5ATP9, a gene up-regulated by HCV NS5A protein

Cancer Lett. 2008 Feb 8;259(2):192-7. doi: 10.1016/j.canlet.2007.10.029.

Abstract

Non-structural protein 5A (NS5A) appears to interact with a variety of cellular proteins and play an important role in mediating cell growth, cellular signaling pathways and pathogenesis of hepatitis C virus (HCV). NS5ATP9 was identified as a NS5A trans-activated protein in suppression subtractive hybridization (SSH), and the regulation was confirmed by luciferase reporter assay and quantitative real time PCR (qRT-PCR). A minimal promoter region contained within the 211bp (nucleotides -161 to +50bp) immediately upstream of the transcription initiation site. NS5ATP9 is a NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / virology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Tumor
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Gene Expression Regulation, Neoplastic*
  • Gene Library
  • Genes, Reporter
  • Hepacivirus / genetics
  • Hepacivirus / metabolism
  • Hepacivirus / pathogenicity*
  • Hepatitis C / complications*
  • Hepatitis C / genetics
  • Hepatitis C / metabolism
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / virology
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • RNA, Messenger / metabolism
  • Transcriptional Activation
  • Transfection
  • Up-Regulation
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism*

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • PCLAF protein, human
  • RNA, Messenger
  • Viral Nonstructural Proteins
  • NS-5 protein, hepatitis C virus