Abstract
Non-structural protein 5A (NS5A) appears to interact with a variety of cellular proteins and play an important role in mediating cell growth, cellular signaling pathways and pathogenesis of hepatitis C virus (HCV). NS5ATP9 was identified as a NS5A trans-activated protein in suppression subtractive hybridization (SSH), and the regulation was confirmed by luciferase reporter assay and quantitative real time PCR (qRT-PCR). A minimal promoter region contained within the 211bp (nucleotides -161 to +50bp) immediately upstream of the transcription initiation site. NS5ATP9 is a NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / metabolism*
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Carcinoma, Hepatocellular / virology
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line, Tumor
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Cloning, Molecular
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DNA-Binding Proteins
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Gene Expression Regulation, Neoplastic*
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Gene Library
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Genes, Reporter
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Hepacivirus / genetics
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Hepacivirus / metabolism
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Hepacivirus / pathogenicity*
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Hepatitis C / complications*
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Hepatitis C / genetics
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Hepatitis C / metabolism
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Humans
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism*
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Liver Neoplasms / virology
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Molecular Sequence Data
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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RNA, Messenger / metabolism
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Transcriptional Activation
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Transfection
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Up-Regulation
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism*
Substances
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Carrier Proteins
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DNA-Binding Proteins
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PCLAF protein, human
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RNA, Messenger
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Viral Nonstructural Proteins
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NS-5 protein, hepatitis C virus