Decolorization of cotton pulp black liquor by Pleurotus ostreatus B1 in a bubble-column reactor (BCR) was studied. The optimal conditions for the running of BCR are 30 degrees C, pH 6.0, aeration rate 1.2 L min(-1), and mycelial age 7 days. Under the optimal conditions, the BCR was run for four cycles (each cycle, 12 days) and the same mycelial pellets were reused. The ultimate decolorization and COD removal rates are 76% and 80%, respectively.