Abstract
To characterize the contributions of individual amino acids to the structure or function of a protein, researchers have adopted directed evolution approaches, which use iterated cycles of mutagenesis and selection or screening to search vast areas of sequence space for sets of mutations that provide insights into the protein of interest.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Directed Molecular Evolution / methods
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Directed Molecular Evolution / trends*
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Evolution, Molecular*
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Mutagenesis, Site-Directed / methods
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Mutagenesis, Site-Directed / trends*
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Protein Engineering / methods
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Protein Engineering / trends*
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Proteins / chemistry*
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Proteins / genetics*
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Structure-Activity Relationship