S-(4-Nitrophenacyl)glutathione is a specific substrate for glutathione transferase omega 1-1

Anal Biochem. 2008 Mar 1;374(1):25-30. doi: 10.1016/j.ab.2007.09.029. Epub 2007 Sep 29.

Abstract

Glutathione transferase omega 1-1 (GSTO1-1) catalyzes the biotransformation of arsenic and is implicated as a factor influencing the age-at-onset of Alzheimer's disease and the posttranslational activation of interleukin 1beta (IL-1beta). Investigation of the biological role of GSTO1-1 variants has been hampered by the lack of a specific assay for GSTO1-1 activity in tissue samples that contain other GSTs and other enzymes with similar catalytic specificities. Previous studies (P. G. Board and M. W. Anders, Chem. Res. Toxicol. 20 (2007) 149-154) have shown that GSTO1-1 catalyzes the reduction of S-(phenacyl)glutathiones to acetophenones. A new substrate, S-(4-nitrophenacyl)glutathione (4NPG), has been prepared and found to have a high turnover with GSTO1-1 but negligible activity with GSTO2-2 and other members of the glutathione transferase superfamily. A spectrophotometric assay with 4NPG as a substrate has been used to determine GSTO1-1 activity in several human breast cancer cell lines and in mouse liver and brain tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / enzymology
  • Cell Line, Tumor
  • Glutathione / analogs & derivatives*
  • Glutathione / metabolism
  • Glutathione Transferase / metabolism*
  • Humans
  • Mice
  • Spectrophotometry, Ultraviolet

Substances

  • S-(4-nitrophenacyl)glutathione
  • GSTO1 protein, human
  • Glutathione Transferase
  • Glutathione