Objective: To investigate the chemical constituents of effective part of Epimedium brevicornum.
Method: The sample was extracted with ethanol and purified by macroporous resin. The structures were identified by HPLC-MS3 experiments.
Result: Nine compounds were identified from the effective part of E. brevicornum.
Conclusion: The method is simple and rapid for the identification of the flavonoids from E. brevicornum.