Implementation of electrochemiluminescence microanalysis in PCB technology

Annu Int Conf IEEE Eng Med Biol Soc. 2007:2007:2944-7. doi: 10.1109/IEMBS.2007.4352946.

Abstract

We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro-(10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrochemistry
  • Electronics
  • Hydrogen Peroxide / chemistry
  • Luminescent Agents / chemistry
  • Luminescent Measurements / instrumentation
  • Luminescent Measurements / methods*
  • Luminol / chemistry
  • Microelectrodes
  • Microfluidic Analytical Techniques*
  • Silver / chemistry
  • Silver Compounds / chemistry

Substances

  • Luminescent Agents
  • Silver Compounds
  • Silver
  • Luminol
  • Hydrogen Peroxide
  • silver chloride