Abstract
We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Animals
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Artificial Intelligence
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Embryo, Nonmammalian / cytology*
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Image Enhancement / methods
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Image Interpretation, Computer-Assisted / methods*
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Imaging, Three-Dimensional / methods
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Microscopy, Confocal / methods*
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Microscopy, Fluorescence / methods*
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Pattern Recognition, Automated / methods*
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Reproducibility of Results
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Sensitivity and Specificity
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Zebrafish / anatomy & histology*
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Zebrafish / embryology*