Abstract
The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Cations
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Chlorocebus aethiops
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Circular Dichroism
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DNA / administration & dosage
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DNA / chemistry*
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Dimyristoylphosphatidylcholine
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Liposomes
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Polyethylene Glycols*
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Quaternary Ammonium Compounds
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Surface-Active Agents
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Transfection*
Substances
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Cations
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Liposomes
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Quaternary Ammonium Compounds
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Surface-Active Agents
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poly(ethylene glycol) monolaurate
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Green Fluorescent Proteins
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Polyethylene Glycols
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DNA
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Dimyristoylphosphatidylcholine