Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity

Martin D Witte; Carlos V Descals; Sebastiaan V P de Lavoir; Bogdan I Florea; Gijsbert A van der Marel; Herman S Overkleeft

doi:10.1039/b711531h

Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity

Org Biomol Chem. 2007 Nov 21;5(22):3690-7. doi: 10.1039/b711531h. Epub 2007 Aug 29.

Authors

Martin D Witte¹, Carlos V Descals, Sebastiaan V P de Lavoir, Bogdan I Florea, Gijsbert A van der Marel, Herman S Overkleeft

Affiliation

¹ Bioorganic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.

PMID: 17971999
DOI: 10.1039/b711531h

Abstract

In this paper the development of a fluorescent activity based probe, Bodipy-VAD-Fmk, for visualization of yeast peptide N-glycanase is described. The activity based probe is used to assess the efficacy of known and new chitobiose-based electrophilic traps to bind yeast peptide N-glycanase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Chloromethyl Ketones / chemistry
Amino Acid Chloromethyl Ketones / metabolism*
Fluorescence
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism*
Saccharomyces cerevisiae / enzymology*

Substances

Amino Acid Chloromethyl Ketones
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase