[Evaluation of the transcription of locus rfb for the biosynthesis of LPS in Leptospira interrogans subtype hardjoprajitno]

Rev Cubana Med Trop. 2005 Jan-Apr;57(1):43-4.
[Article in Spanish]

Abstract

Plasmid pGL4W15-96 was constructed from the pGL4W74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed J15 promoter with the objective of confirming the role of J15 sequence as a promoter for Leptospira, which restored the transcription of the gene of resistance to kanamycin in Escherichia coli and Leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacterial Proteins*
  • Drug Resistance, Bacterial / genetics*
  • Escherichia coli / drug effects
  • Kanamycin / pharmacology*
  • Leptospira interrogans / drug effects*
  • Leptospira interrogans / genetics*
  • Leptospira interrogans / metabolism
  • Lipopolysaccharides / biosynthesis*
  • O Antigens
  • Plasmids / genetics*
  • Promoter Regions, Genetic / genetics
  • Transcription, Genetic*

Substances

  • Bacterial Proteins
  • Lipopolysaccharides
  • O Antigens
  • rfb protein, Bacteria
  • Kanamycin