An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model

Oscar Cruciani; Luisa Mannina; Anatoli P Sobolev; Cesare Cametti; AnnaLaura Segre

doi:10.3390/11050334

An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model

Molecules. 2006 May 10;11(5):334-44. doi: 10.3390/11050334.

Authors

Oscar Cruciani¹, Luisa Mannina, Anatoli P Sobolev, Cesare Cametti, AnnaLaura Segre

Affiliation

¹ Istituto di Metodologie Chimiche, CNR, 00016 Monterotondo Stazione, Roma, Italy.

Abstract

In this paper we report a comparative characterization of Small Unilamellar Vesicles (SUVs), Large Unilamellar Vesicles (LUVs) and Multilamellar Vesicles (MLVs) prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine (POPC), carried out using two NMR techniques, namely High Resolution NMR in solution and High Resolution-Magic Angle Spinning (HR-MAS). The size and size distributions of these vesicles were investigated using the dynamic light scattering technique. An improved assignment of the (1)H-NMR spectrum of MLVs is also reported.

MeSH terms

Light
Liposomes*
Magnetic Resonance Spectroscopy / methods*
Models, Chemical*
Molecular Structure
Phosphatidylcholines / chemistry*
Scattering, Radiation

Substances

Liposomes
Phosphatidylcholines
1-palmitoyl-2-oleoylphosphatidylcholine