Abstract
The very C-terminus of c-Src is a ligand for PDZ domains. In a screen for PDZ domains that interact with c-Src, we identified one of the PDZ domains of the Ligand-of-Numb protein X1 (LNX1), a multiple PDZ domain scaffold and RING type E3 ubiquitin ligase. We demonstrate that the interaction of c-Src with LNX1 depends on the C-terminal PDZ ligand of c-Src. Furthermore, we show that c-Src phosphorylates LNX1. Moreover, c-Src itself is ubiquitinated by LNX1, suggesting an interdependent regulation of c-Src and LNX1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CSK Tyrosine-Protein Kinase
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Cell Line
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Humans
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Ligands
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PDZ Domains*
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Phosphorylation
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Protein Interaction Mapping
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Protein-Tyrosine Kinases / analysis
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Protein-Tyrosine Kinases / metabolism*
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Substrate Specificity
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Ubiquitin / metabolism
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Ubiquitin-Protein Ligases / analysis
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Ubiquitin-Protein Ligases / metabolism*
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src-Family Kinases
Substances
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Ligands
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Ubiquitin
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LNX1 protein, human
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Ubiquitin-Protein Ligases
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Protein-Tyrosine Kinases
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CSK Tyrosine-Protein Kinase
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src-Family Kinases
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CSK protein, human