Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

S L Yu; H J Chung; B C Sang; C S Park; J H Lee; D H Yoon; S H Lee; K D Choi

doi:10.1080/10495390701413391

Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray

Anim Biotechnol. 2007;18(4):275-85. doi: 10.1080/10495390701413391.

Authors

S L Yu¹, H J Chung, B C Sang, C S Park, J H Lee, D H Yoon, S H Lee, K D Choi

Affiliation

¹ Division of Animal Science and Resources, Chungnam National University, Daejeon, Korea.

PMID: 17934901
DOI: 10.1080/10495390701413391

Abstract

The 788-gene microarray was manufactured using selected elements from three different cDNA libraries in order to identify molecular processes that determine phenotypic characteristics between loin (M. longissimus thoracis) and round (M. semimembranosus) muscles. Microarray analyses identified 24 differentially expressed genes between the two muscles investigated. Five of the genes were verified by quantitative RT-PCR and three of them were mapped on bovine chromosomes using 5,000 rad bovine radiation hybrid (RH) panel. The map locations indicated that they were mapped in the same chromosomal regions where IMF and growth QTLs were located, suggesting that they are most possible positional candidate genes for the traits.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle / genetics*
Cattle / metabolism
Gene Expression
Gene Expression Profiling / methods
Gene Expression Profiling / veterinary
Meat
Muscle Fibers, Skeletal / physiology*
Muscle, Skeletal / physiology*
Oligonucleotide Array Sequence Analysis / veterinary
Radiation Hybrid Mapping / veterinary