Expression of a bacterial alpha-amylase gene in transgenic rice seeds

Transgenic Res. 2008 Aug;17(4):645-50. doi: 10.1007/s11248-007-9144-5. Epub 2007 Oct 10.

Abstract

An alpha-amylase gene from Bacillus stearothermophilus under the control of the promoter of a major rice-seed storage protein was introduced into rice. The transgenic line with the highest alpha-amylase activity reached about 15,000 U/g of seeds (one unit is defined as the amount of enzyme that produces 1 mumol of reducing sugar in 1 min at 70 degrees C). The enzyme produced in the seeds had an optimum pH of 5.0-5.5 and optimum temperature of 60-70 degrees C. Without extraction or purification, the power of transgenic rice seeds was able to liquify 100 times its weight of corn powder in 2 h. Thus, the transgenic rice could be used for industrial starch liquefaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Geobacillus stearothermophilus / enzymology*
  • Geobacillus stearothermophilus / genetics
  • Hydrogen-Ion Concentration
  • Oryza / enzymology*
  • Oryza / genetics
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism*
  • Plasmids
  • Starch / metabolism
  • Temperature
  • Transformation, Genetic
  • alpha-Amylases / genetics*
  • alpha-Amylases / isolation & purification
  • alpha-Amylases / metabolism*

Substances

  • Starch
  • alpha-Amylases