Abstract
This study evaluated the potential of a Leishmania antigen vaccine in protecting BALB/c mice against Leishmania chagasi. Mice received two subcutaneous doses of L. amazonensis vaccine with Corynebacterium parvum and subsequent boost was done without adjuvant. One week later, mice were challenged with L. chagasi. We observed that this vaccine caused a significant reduction in parasite load in liver and spleen and induced a high production of IFN-gamma and IL-4 by spleen cells from vaccinated mice in response to Leishmania antigen. Together, our data show that this vaccine is capable of inducing a Th1/Th2 response that is important to control parasite replication.
MeSH terms
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Animals
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Antigens, Protozoan / immunology*
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Female
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Interferon-gamma / biosynthesis
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Interleukin-4 / biosynthesis
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Leishmania / immunology*
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Leishmaniasis / immunology*
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Leishmaniasis / parasitology
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Leishmaniasis / prevention & control
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Leishmaniasis Vaccines / administration & dosage
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Leishmaniasis Vaccines / immunology*
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Leishmaniasis Vaccines / therapeutic use
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Liver / drug effects
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Liver / metabolism
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Liver / parasitology
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Mice
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Mice, Inbred BALB C
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Propionibacterium acnes / immunology*
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Spleen / drug effects
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Spleen / metabolism
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Spleen / parasitology
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Th1 Cells / drug effects
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Th1 Cells / immunology
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Th2 Cells / drug effects
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Th2 Cells / immunology
Substances
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Antigens, Protozoan
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Leishmaniasis Vaccines
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Interleukin-4
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Interferon-gamma