The purpose of this study was to clarify whether masticatory movements during chewing of a wax cube associate with food mixing ability. Twenty-six dentate subjects (mean age 25.3 years) were directed to chew a two-coloured paraffin wax cube for 10 strokes on preferred chewing side. Mixing Ability Index (MAI) was determined from the colour mixture and shape of the chewed wax cube. MAI was employed as an estimate of food mixing ability. Mandibular movements during chewing of the wax cube were recorded using a six-degrees-of freedom jaw movements recording system, and motion of a lower incisal point of the mandible was computed. Twelve parameters of masticatory movements in relation to mandibular excursion, angle, velocity, cycle duration, duration of each phase were measured for each masticatory cycle. In addition, unilateral maximum biting was performed, and the maximum force during biting was measured using a force-transducer. A multiple regression analysis identified vertical amplitude, closing duration, closing angle and maximum bite force as significant predictors accounting for 63% of inter-subject variation (adjusted R(2)) in the MAI (P < 0.05). Only some parameters of masticatory movements seem to have an influence on the MAI in dentate subjects.