Abstract
Aim:
To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.
Methodology:
Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.
Results:
SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.
Conclusion:
Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily G, Member 2
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ATP-Binding Cassette Transporters / biosynthesis*
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ATP-Binding Cassette Transporters / pharmacology
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Actins / biosynthesis
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Adolescent
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Adult
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Benzimidazoles
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Cell Differentiation
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Cell Separation
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Cells, Cultured
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Dental Pulp / cytology*
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Dental Pulp / metabolism
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Extracellular Matrix Proteins / biosynthesis
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Fluorescent Dyes
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Gene Expression
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Humans
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Intermediate Filament Proteins / biosynthesis
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Neoplasm Proteins / biosynthesis*
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Nerve Tissue Proteins / biosynthesis
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Nestin
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Odontoblasts / metabolism*
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Phosphoproteins / biosynthesis
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Receptor, Notch1 / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction
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Sialoglycoproteins
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Stem Cells / drug effects
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Stem Cells / metabolism*
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Verapamil / pharmacology
Substances
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ABCG2 protein, human
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ATP Binding Cassette Transporter, Subfamily G, Member 2
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ATP-Binding Cassette Transporters
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Actins
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Benzimidazoles
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DMP1 protein, human
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Extracellular Matrix Proteins
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Fluorescent Dyes
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Intermediate Filament Proteins
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NES protein, human
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Neoplasm Proteins
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Nerve Tissue Proteins
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Nestin
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Phosphoproteins
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Receptor, Notch1
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Sialoglycoproteins
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dentin sialophosphoprotein
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Verapamil
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bisbenzimide ethoxide trihydrochloride