Side population cells expressing ABCG2 in human adult dental pulp tissue

Int Endod J. 2007 Dec;40(12):949-58. doi: 10.1111/j.1365-2591.2007.01301.x. Epub 2007 Oct 4.

Abstract

Aim: To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.

Methodology: Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.

Results: SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.

Conclusion: Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters / biosynthesis*
  • ATP-Binding Cassette Transporters / pharmacology
  • Actins / biosynthesis
  • Adolescent
  • Adult
  • Benzimidazoles
  • Cell Differentiation
  • Cell Separation
  • Cells, Cultured
  • Dental Pulp / cytology*
  • Dental Pulp / metabolism
  • Extracellular Matrix Proteins / biosynthesis
  • Fluorescent Dyes
  • Gene Expression
  • Humans
  • Intermediate Filament Proteins / biosynthesis
  • Neoplasm Proteins / biosynthesis*
  • Nerve Tissue Proteins / biosynthesis
  • Nestin
  • Odontoblasts / metabolism*
  • Phosphoproteins / biosynthesis
  • Receptor, Notch1 / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sialoglycoproteins
  • Stem Cells / drug effects
  • Stem Cells / metabolism*
  • Verapamil / pharmacology

Substances

  • ABCG2 protein, human
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters
  • Actins
  • Benzimidazoles
  • DMP1 protein, human
  • Extracellular Matrix Proteins
  • Fluorescent Dyes
  • Intermediate Filament Proteins
  • NES protein, human
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Nestin
  • Phosphoproteins
  • Receptor, Notch1
  • Sialoglycoproteins
  • dentin sialophosphoprotein
  • Verapamil
  • bisbenzimide ethoxide trihydrochloride