Abstract
Secretion of the cytokine interleukin-2 (IL-2) was investigated in Lactococcus lactis using the secretory machinery of the bacteriocin lactococcin A. Surprisingly, the lcnCD transport genes were not essential for mouse IL-2 secretion. Furthermore, expression of a mature mouse IL-2 gene resulted in interleukin secretion without the requirement for a leader sequence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteriocins / genetics
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Bacteriocins / metabolism
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Blotting, Western
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Interleukin-2 / metabolism*
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L-Lactate Dehydrogenase / genetics
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L-Lactate Dehydrogenase / metabolism
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Lactococcus lactis / genetics
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Lactococcus lactis / metabolism*
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Mice
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Nisin / genetics*
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Promoter Regions, Genetic / genetics
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Protein Sorting Signals / genetics*
Substances
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Bacteriocins
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Interleukin-2
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Protein Sorting Signals
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lactococcin A
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Nisin
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L-Lactate Dehydrogenase