Nisin-controlled extracellular production of interleukin-2 in Lactococcus lactis strains, without the requirement for a signal peptide sequence

Appl Environ Microbiol. 2007 Dec;73(23):7781-4. doi: 10.1128/AEM.01247-07. Epub 2007 Sep 28.

Abstract

Secretion of the cytokine interleukin-2 (IL-2) was investigated in Lactococcus lactis using the secretory machinery of the bacteriocin lactococcin A. Surprisingly, the lcnCD transport genes were not essential for mouse IL-2 secretion. Furthermore, expression of a mature mouse IL-2 gene resulted in interleukin secretion without the requirement for a leader sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriocins / genetics
  • Bacteriocins / metabolism
  • Blotting, Western
  • Interleukin-2 / metabolism*
  • L-Lactate Dehydrogenase / genetics
  • L-Lactate Dehydrogenase / metabolism
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism*
  • Mice
  • Nisin / genetics*
  • Promoter Regions, Genetic / genetics
  • Protein Sorting Signals / genetics*

Substances

  • Bacteriocins
  • Interleukin-2
  • Protein Sorting Signals
  • lactococcin A
  • Nisin
  • L-Lactate Dehydrogenase