Abstract
Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple nonstandard as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acids / chemistry
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Amino Acids / metabolism*
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Amino Acids / toxicity*
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / metabolism*
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Gene Expression Regulation, Bacterial
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Leucine-tRNA Ligase / metabolism*
Substances
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Amino Acids
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Escherichia coli Proteins
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Leucine-tRNA Ligase