From the bark of Zygophyllum fabago L., a new 27-nor-triterpenoid glycoside, 3-O-beta-D-glucopyranosyl-pyrocincholate (1), together with five known compounds, 3-O-6-deoxy-beta-D-glucopyranosyl-pyrocincholate (2), quinovic acid (3), 3-O-6-deoxy-beta-D-glucopyranosyl-quinovic acid (4), 3-O-beta-D-glucopyranosyl- quinovic acid (5) and 3-O-6-deoxy-beta-D-glucopyranosyl-cincholic acid (6), were isolated and their structures elucidated on the basis of spectroscopic data. Compounds 1, 2 and 3 showed some anti-tumour activities by MTT assay.