Rapid determination of viral RNA sequences in mosquitoes collected in the field

Yuki Kihara; Tomomitsu Satho; Yuki Eshita; Kouji Sakai; Akira Kotaki; Tomohiko Takasaki; Yupha Rongsriyam; Narumon Komalamisra; Raweewan Srisawat; Parichat Lapcharoen; Suchada Sumroiphon; Shiroh Iwanaga; Hiroshi Ushijima; Daiji Endoh; Takeshi Miyata; Akira Sakata; Nobuhiro Kashige; Fumio Miake; Shuetsu Fukushi; Masayuki Saijo; Ichiro Kurane; Shigeru Morikawa; Tetsuya Mizutani

doi:10.1016/j.jviromet.2007.07.008

Rapid determination of viral RNA sequences in mosquitoes collected in the field

J Virol Methods. 2007 Dec;146(1-2):372-4. doi: 10.1016/j.jviromet.2007.07.008. Epub 2007 Sep 17.

Affiliation

¹ Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.

PMID: 17869351
DOI: 10.1016/j.jviromet.2007.07.008

Abstract

A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aedes / virology*
Animals
Cell Line
Dengue Virus / genetics
Dengue Virus / isolation & purification*
Female
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Severe Dengue / virology
Thailand

Substances

RNA, Viral