[Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene]

K V Lobanov; N V Korol'kova; S Iu Eremina; L Errais Lopes; S A Proshkin; A S Mironov

[Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene]

Genetika. 2007 Jun;43(6):859-64.

[Article in Russian]

Authors

K V Lobanov, N V Korol'kova, S Iu Eremina, L Errais Lopes, S A Proshkin, A S Mironov

PMID: 17853814

Abstract

Site-directed mutagenesis was used to study the leader region of the Bacillus subtilis pbuE gene, which codes for adenine-specific sensor RNA. Two nucleotide substitutions, 70U --> C and A100 --> G, in the conserved region (A box) of the leader changed the specificity of the sensor RNA in vivo: guanine acted as a positive transcription effector in place of adenine.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Base Sequence
Genes, Bacterial*
Molecular Sequence Data
Mutation*
Nucleic Acid Conformation
RNA, Bacterial / genetics*

Substances

RNA, Bacterial