Oligotyping of celiac multiplex families with the 11th International Histocompatibility Workshop reagents

Tissue Antigens. 1991 Nov;38(5):238-9. doi: 10.1111/j.1399-0039.1991.tb01905.x.

Abstract

Using PCR and SSO probes from the 11th International Histocompatibility Workshop, we oligotyped for HLA-DRB1 gene and DQA1*0501, DQB1*0201 alleles 10 celiac families each with 2 affected children. All families belong to the Italian population except for one, whose mother is originally from Cape Verde island. 8/10 sibling pairs share the DQA1*0501/B1*0201 heterodimer, inherited in cis or in trans arrangement. All the dimer-negative patients were DR4-positive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Celiac Disease / genetics*
  • Celiac Disease / immunology
  • Child
  • Disease Susceptibility / immunology
  • Female
  • Genes, MHC Class II*
  • Genetic Predisposition to Disease
  • HLA-DR Antigens / genetics*
  • Histocompatibility Testing*
  • Humans
  • Italy
  • Male
  • Oligonucleotide Probes
  • Polymerase Chain Reaction

Substances

  • HLA-DR Antigens
  • Oligonucleotide Probes